Examining the involvement of Slx5 in the apoptotic response to chronic activation of the spindle assembly checkpoint
Küçük Resim Yok
Tarih
2019
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
TUBITAK SCIENTIFIC & TECHNICAL RESEARCH COUNCIL TURKEY
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Microtubule-targeting agents represent one of the most successful groups of anticancer drugs used in cancer therapy today. These drugs induce a prolonged mitotic arrest through chronic spindle assembly checkpoint (SAC) activation. Apoptosis, an outcome of the prolonged mitotic arrest, is the main mechanism by which these anticancer drugs kill cancer cells. However, not much is known about the mechanism that directs chronic SAC activation to apoptosis among other possible outcomes. The aim of this study is to investigate whether Slx5, a sumo-targeted ubiquitin E3 ligase, is involved in directing chronic SAC activation to apoptosis. We show that chronic SAC activation triggered by a 10-h nocodazole incubation leads to a prolonged mitotic arrest in the slx5 Delta strain similar to wild type (WT). However, the proportion of cells displaying apoptotic features such as nuclear fragmentation, DNA fragmentation, and reactive oxygen species (ROS) production were increased more in the WT strain during the chronic SAC activation compared to slx5 Delta, indicating that SLx5 may be involved in the chronic SAC-activation-apoptosis relation. We also showed that the possible role of Slx5 in the chronic SAC activation-apoptosis association was not through ubiquitin dependent degradation of 3 apoptosis-related and sumoylated candidate proteins.
Açıklama
Anahtar Kelimeler
Saccharomyces cerevisiae, spindle assembly checkpoint, cancer, prolonged mitotic arrest, apoptosis
Kaynak
TURKISH JOURNAL OF BIOLOGY
WoS Q Değeri
Q4
Scopus Q Değeri
Q2
Cilt
43
Sayı
3
