Inhibition of voltage?gated potassium channels affect expressions of miR-126 and miR-126* in breast cancer cell lines

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dc.authorid0000-0003-3771-3277en_US
dc.contributor.authorTurgut Coşan, Didem
dc.contributor.authorSoyocak, Ahu
dc.contributor.authorÖner, Çağrı
dc.date.accessioned2024-07-12T21:02:11Z
dc.date.available2024-07-12T21:02:11Z
dc.date.issued2020en_US
dc.departmentFakülteler, Tıp Fakültesien_US
dc.description.abstractAIM: We aimed to determine the possible correlation between voltage?gated potassium channels and micro RNAs in breast cancer and metastatic breast cancer cells. METHOD: Kv1.3 and Kv10.1 channels were inhibited by specific siRNAs using a lipofectamine-based transfection in MCF-7 and MDA-MB-231 cells. After transfection, total RNA was isolated, and then miR-126 and miR-126* expressions were observed using RT-PCR. RESULTS: There was a negative correlation between Kv channels and miRNAs according to the characteristics of the breast cancer cells. The inhibition was observed not only in Kv1.3 but also in Kv10.1 in MCF-7 cells, and miR-126 and miR-126* expressions were downregulated compared to the control group (p < 0.001). The inhibition of these channels in MDA-MB-231 cells caused an upregulation of miR-126 and miR-126* expressions (p < 0.001). CONCLUSION: The miR-126 and miR-126* expressions differed according to benign and malign breast cancer cell lines. Furthermore, we found that miR-126/126* may interact with Kv1.3 and Kv10.1 voltage-gated potassium channels. Our study suggests and indicates the relationship between Kv channels and miRNAs in breast cancer cells (Tab. 1, Fig. 2, Ref. 51).en_US
dc.identifier.citationTurgut Coşan, D., Soyocak, A. ve Öner, Ç. (2020). Inhibition of voltage?gated potassium channels affect expressions of miR-126 and miR-126* in breast cancer cell lines. Bratislava Medical Journal, AEPress. 121(2), s. 111-116.en_US
dc.identifier.doi10.4149/BLL_2020_014
dc.identifier.endpage116en_US
dc.identifier.issn1336-0345
dc.identifier.issue2en_US
dc.identifier.pmid32115962en_US
dc.identifier.scopus2-s2.0-85080840671en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage111en_US
dc.identifier.urihttp://www.elis.sk/index.php?page=shop.product_details&flypage=flypage.tpl&product_id=6525&category_id=158&option=com_virtuemart&vmcchk=1&Itemid=1
dc.identifier.urihttps://doi.prg/10.4149/BLL_2020_014
dc.identifier.urihttps://hdl.handle.net/20.500.12415/3592
dc.identifier.volume121en_US
dc.identifier.wosWOS:000508930100002en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorÖner, Çağrı
dc.language.isoenen_US
dc.publisherAEPressen_US
dc.relation.ispartofBratislava Medical Journalen_US
dc.relation.publicationcategoryUluslararası Hakemli Dergide Makale - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzKY00447
dc.subjectbreast canceren_US
dc.subjectKv1.3en_US
dc.subjectKv10.1en_US
dc.subjectmiR-126en_US
dc.subjectmiR-126*en_US
dc.titleInhibition of voltage?gated potassium channels affect expressions of miR-126 and miR-126* in breast cancer cell linesen_US
dc.typeArticle
dspace.entity.typePublication

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