Collagen type I induces a balance in the expression of anti- and pro-apoptotic genes in hepatocellular carcinoma cells

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dc.authorid0000-0002-4605-1167en_US
dc.authorid0000-0001-9801-1506en_US
dc.authorid0000-0002-4451-6813en_US
dc.authorid0000-0003-0674-090Xen_US
dc.authorid0000-0002-4474-7371en_US
dc.date.accessioned2024-07-12T21:10:15Z
dc.date.available2024-07-12T21:10:15Z
dc.date.issued2022en_US
dc.departmentFakülteler, Tıp Fakültesien_US
dc.description.abstractBackground? The importance of extracellular matrix (ECM) components in the progression of hepatocellular carcinoma (HCC) has been shown in many studies. Although restoring or activating apoptosis in tumors is an active area of cancer research, little is known regarding the effects of collagen type I, the main ECM component in the liver, on apoptosis of HCC cells. Here, we investigated the apoptotic profiles of HCC cells in a microenvironment with collagen type I. Method? In this in vitro study, we assessed the effects of collagen type I on HepG2 cells in preconfluent and confluent states. We determined the mRNA levels of 25 genes, which are the key players of apoptosis. Flow cytometry-based apoptosis detection was performed by use ofAnnexin V/PI staining. Confocal laser scanning microscopy was used to assess P53 immunofluorescence in the cells. Results? The microenvironment with collagen type I and the confluency state of HepG2 cells affected the expression of 13 genes involved in apoptosis. We observed no significant change in the number of cells undergoing apoptosis depending on the confluency state or the presence of collagen type I. P53 immunofluorescence demonstrated no significant changes. Conclusion? We propose an apoptotic balance concerning overall cell survival which might be caused by the counteraction of positive and negative mediators of apoptosis. This study might provide data for the involvement of collagen type I in apoptotic responses of HCC, and contribute to a better understanding of cancer microenvironment.en_US
dc.identifier.citationCakil Y.D, Akbulut Z, Maras H, Kayali D.G, Aktas R.G. Collagen type I induces a balance in the expression of anti- and pro-apoptotic genes in hepatocellular carcinoma cells. Middle East J Cancer. 2022;13(1):89-98. doi: 10. 30476/mejc.2021.85875.1312.en_US
dc.identifier.doi10.30476/MEJC.2021.85875.1312
dc.identifier.endpage98en_US
dc.identifier.issn2008-6687
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-85125644857en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage89en_US
dc.identifier.urihttps://mejc.sums.ac.ir/article_47587.html
dc.identifier.urihttps://doi.prg/10.30476/MEJC.2021.85875.1312
dc.identifier.urihttps://hdl.handle.net/20.500.12415/4225
dc.identifier.volume13en_US
dc.identifier.wosWOS:000763019300004en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorDönmez Çakıl, Yaprak
dc.institutionauthorAkbulut, Zeynep
dc.institutionauthorMaraş, Hatice
dc.institutionauthorKayalı, Damla
dc.institutionauthorAktaş, Ranan Gülhan
dc.language.isoenen_US
dc.publisherShiraz University of Medical Sciencesen_US
dc.relation.ispartofMiddle East Journal of Canceren_US
dc.relation.publicationcategoryUluslararası Hakemli Dergide Makale - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzKY02951
dc.subjectCanceren_US
dc.subjectCell deathen_US
dc.subjectExtracellular matrixen_US
dc.subjectHepG2en_US
dc.subjectMicroenvironmenten_US
dc.titleCollagen type I induces a balance in the expression of anti- and pro-apoptotic genes in hepatocellular carcinoma cellsen_US
dc.typeArticle
dspace.entity.typePublication

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