PIWI interacting rna-823: epigenetic regulator of the triple negative breast cancer cells proliferation

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dc.authorid0000-0003-3771-3277en_US
dc.contributor.authorÖner, Çağrı
dc.contributor.authorÇolak, Ertuğrul
dc.date.accessioned2024-07-12T21:03:33Z
dc.date.available2024-07-12T21:03:33Z
dc.date.issued2022en_US
dc.departmentFakülteler, Tıp Fakültesien_US
dc.description.abstractObjectives: Triple negative breast cancer cells are estrogen, progesterone, and HER receptor negative, malign breast cancer cells. These cells have high telomerase activity, and this activity gives these cells high proliferation and evading apoptosis abilities. In this study, we aimed to determine the affect piR-823 on genetic parameters of proliferation and ER? status in MDA-MB-231 cells. Methods: After anti-piR-823 transfection, proliferation of cells was tested by XTT. Gene expressions were determined by RT-PCR. Protein expressions were determined by ELISA. Results: The proliferation decreased after inhibition (p<0.001). Gene and protein expressions of ER? were upregulated while hTERT was downregulated after inhibition (p<0.001). Furthermore, piR-823 inhibition cause to decrease PI3K/ AKT/mTOR gene expressions and miR-126 expression (p<0.001). Conclusion: Obtained data indicates that piR-823 inhibition lead MDA-MB-231 cells to increase ER? expression. Decreased expressions of hTERT and PI3K/AKT/mTOR pathway affect cell proliferation. Moreover, miR-126 decrease indicates that piRNAs and miRNAs can share the same target molecules and piRNA expression changes might have an impact on miRNA expressions. All obtained data is important on the perspective of piRNAs and their effect on cellular characteristics of triple negative breast cancer cells.en_US
dc.identifier.citationOner, C. ve Colak, E. (2022).PIWI interacting rna-823: epigenetic regulator of the triple negative breast cancer cells proliferation. EJMO, Kare Publishing. 6(4), s. 339-344.en_US
dc.identifier.doi10.14744/ejmo.2022.12505
dc.identifier.endpage344en_US
dc.identifier.issn2587-196X
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-85146247268en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage339en_US
dc.identifier.trdizinid1172761en_US
dc.identifier.urihttps://www.ejmo.org/10.14744/ejmo.2022.12505/
dc.identifier.urihttps://doi.prg/10.14744/ejmo.2022.12505
dc.identifier.urihttps://hdl.handle.net/20.500.12415/3679
dc.identifier.volume6en_US
dc.identifier.wosWOS:000906412400008en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.institutionauthorÖner, Çağrı
dc.language.isoenen_US
dc.publisherKare Publishingen_US
dc.relation.ispartofEurasian Journal of Medicine and Oncologyen_US
dc.relation.publicationcategoryUlusal Hakemli Dergide Makale - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzKY00829
dc.subjectPhosphatidylinositol-3-Kinaseen_US
dc.subjectpiR-823en_US
dc.subjectTriple Negative Breast Canceren_US
dc.titlePIWI interacting rna-823: epigenetic regulator of the triple negative breast cancer cells proliferationen_US
dc.typeArticle
dspace.entity.typePublication

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