SLX5 deletion confers tolerance to oxidative stress in Saccharomyces cerevisiae
Küçük Resim Yok
Tarih
2022
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
FEMS Microbiology Letters
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Slx5, a subunit of a SUMO-targeted ubiquitin ligase (STUbL) in yeast, has been implicated in maintenance of genomic stability. SUMOylation is an important post-translational modification involved in the regulation of several important cellular processes and cellular response to various environmental stressors. Oxidative stress occurs when production of reactive oxygen species (ROS) exceeds the antioxidant defense capacity of the cell. Elevated ROS levels cause oxidative damage to important cellular macromolecules such as DNA, lipids, and proteins, which is associated with several diseases. Herein, we investigated the role of Slx5 in oxidative stress tolerance in Saccharomyces cerevisiae. We show that deletion of SLX5 increases survival of yeast cells in response to H2O2-induced oxidative stress in a cell cycle independent manner. Accumulation of intracellular ROS as well as DNA and lipid damages were reduced; expressions of antioxidant defense mechanism-related genes were increased in slx5 cells compared to wild type (WT) under oxidative stress. We also show that slx5 cells have increased intracellular ROS levels and oxidative damage to DNA and lipids compared to WT in the absence of oxidative stress. Thus, our data together suggest that an adaptive stress induced by SLX5 deletion increases tolerance to oxidative stress in slx5 cells.
Açıklama
Anahtar Kelimeler
Slx5, Oxidative stress tolerance, Adaptive stress
Kaynak
FEMS Microbiology Letters
WoS Q Değeri
Q4
Scopus Q Değeri
Cilt
369
Sayı
Künye
Thomas, P.B., Kaluç, N. and Aybastıer, Ö. (2022). SLX5 deletion confers tolerance to oxidative stress in Saccharomyces cerevisiae. FEMS Microbiology Letters, 369, p.1-8.
