SLX5 deletion confers tolerance to oxidative stress in Saccharomyces cerevisiae

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dc.authorid0000-0001-7627-0291en_US
dc.authorid0000-0002-7323-3150en_US
dc.contributor.authorThomas, Pınar B.
dc.contributor.authorKaluç, Nur
dc.contributor.authorAybastıer, Önder
dc.date.accessioned2024-07-12T21:03:48Z
dc.date.available2024-07-12T21:03:48Z
dc.date.issued2022en_US
dc.departmentFakülteler, Tıp Fakültesien_US
dc.description.abstractSlx5, a subunit of a SUMO-targeted ubiquitin ligase (STUbL) in yeast, has been implicated in maintenance of genomic stability. SUMOylation is an important post-translational modification involved in the regulation of several important cellular processes and cellular response to various environmental stressors. Oxidative stress occurs when production of reactive oxygen species (ROS) exceeds the antioxidant defense capacity of the cell. Elevated ROS levels cause oxidative damage to important cellular macromolecules such as DNA, lipids, and proteins, which is associated with several diseases. Herein, we investigated the role of Slx5 in oxidative stress tolerance in Saccharomyces cerevisiae. We show that deletion of SLX5 increases survival of yeast cells in response to H2O2-induced oxidative stress in a cell cycle independent manner. Accumulation of intracellular ROS as well as DNA and lipid damages were reduced; expressions of antioxidant defense mechanism-related genes were increased in slx5 cells compared to wild type (WT) under oxidative stress. We also show that slx5 cells have increased intracellular ROS levels and oxidative damage to DNA and lipids compared to WT in the absence of oxidative stress. Thus, our data together suggest that an adaptive stress induced by SLX5 deletion increases tolerance to oxidative stress in slx5 cells.en_US
dc.identifier.citationThomas, P.B., Kaluç, N. and Aybastıer, Ö. (2022). SLX5 deletion confers tolerance to oxidative stress in Saccharomyces cerevisiae. FEMS Microbiology Letters, 369, p.1-8.en_US
dc.identifier.doi10.1093/femsle/fnac077
dc.identifier.endpage8en_US
dc.identifier.pmid35981831en_US
dc.identifier.scopus2-s2.0-85138447481en_US
dc.identifier.startpage1en_US
dc.identifier.urihttps://doi.prg/10.1093/femsle/fnac077
dc.identifier.urihttps://hdl.handle.net/20.500.12415/3702
dc.identifier.volume369en_US
dc.identifier.wosWOS:000848326100002en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorThomas, Pınar B.
dc.institutionauthorKaluç, Nur
dc.language.isoenen_US
dc.publisherFEMS Microbiology Lettersen_US
dc.relation.ispartofFEMS Microbiology Lettersen_US
dc.relation.publicationcategoryUluslararası Hakemli Dergide Makale - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzKY00939
dc.subjectSlx5en_US
dc.subjectOxidative stress toleranceen_US
dc.subjectAdaptive stressen_US
dc.titleSLX5 deletion confers tolerance to oxidative stress in Saccharomyces cerevisiaeen_US
dc.typeArticle
dspace.entity.typePublication

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